Roles of RuvC and RecG in Phage λ Red-Mediated Recombination
نویسندگان
چکیده
منابع مشابه
Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG.
ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introduced into ruvA, ruvB, and ruvC strains. Conjugational recombination was reduce...
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Induction of the SOS response in Escherichia coli activates normally repressed DNA replication which is termed inducible stable DNA replication (iSDR). We previously demonstrated that initiation of iSDR requires the products of genes, such as recA, recB and recC, that are involved in the early stages of homologous recombination. By measuring the copy number increase of the origin (oriM1) region...
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The rap gene of bacteriophage was placed in the chromosome of an Escherichia coli K-12 strain in which the recBCD gene cluster had previously been replaced by the red genes and in which the recG gene had been deleted. Recombination between linear double-stranded DNA molecules and the chromosome was tested in variants of the recG red rap strain bearing mutations in genes known to affect recombin...
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The Red recombination system of bacteriophage lambda is widely used for genetic engineering because of its ability to promote recombination between bacterial chromosomes or plasmids and linear DNA species introduced by electroporation. The process is known to be intimately tied to replication, but the cellular functions which participate with Red in this process are largely unknown. Here two su...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1999
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.181.17.5402-5408.1999